Figure 5

Expression of C-type Natriuretic Peptide and its Specific Guanylyl Cyclase-Coupled Receptor in Pig Ovarian Granulosa Cells

Soo Mi Kim, Suhn Hee Kim, Kyung Woo Cho, Sun Young Kim and Sung Zoo Kim*

Published: 22 August, 2018 | Volume 2 - Issue 1 | Pages: 014-025

icci-aid1004-g005

Figure 5:

(A) Dose-dependent cGMP production in response to NPs in the granulosa cell membranes from similar size follicles of the pig ovary. Membranes were prepared by pooling of the granulosa cells from 20 individuals, and incubated in the presence of CNP(1-22) (), BNP(1-26) () and ANP(1-28) (). Basal values (26.30±0.80 pmol/mg protein/min) obtained from the incubation in the absence of NPs were subtracted from each point. The data were expressed as mean ± SE of triplicate determinations. *P<0.05 and **P<0.01 for comparison of CNP(1-22) -treated group vs. BNP(1-26) or ANP(1-28) -treated groups. (B) Effect of HS-142-1 on CNP(1-22) -stimulated cGMP production in the granulosa cell membranes of the pig ovary. Membranes were incubated with 1 µM CNP(1-22) either alone or in the presence of increasing concentrations of HS-142-1. The data were expressed as mean±SE of triplicate determinations. *P<0.05 and **P<0.01 for comparison of CNP(1-22) only-treated group vs. CNP(1-22) plus HS-142-1-treated groups.

Read Full Article HTML DOI: 10.29328/journal.icci.1001004 Cite this Article Read Full Article PDF

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